原始骨血肿的骨再生潜能的实验研究

Experiment Study of the Osteogenetic Potential of Bone Hematoma

目的 探索骨折后冷藏原始骨血肿的骨再生潜能和对骨折愈合的影响。方法 在新西兰兔股骨、胫骨上制造骨缺损模型 ,3d后取出骨血肿并 4℃冷藏 ,3d后冷藏的原始骨血肿回置到实验组骨缺损区 ,定期摄X线片和取出骨痂行组织学观察。结果 实验组从 2、 4、 6周均见明显骨痂形成和骨膜反应 ,组织学观察可见有成骨样细胞形成。结论 冷藏的原始骨血肿仍有很好的骨再生潜能 ,可促进骨折、骨缺损区骨愈合。骨折行手术治疗尤其是闭合性骨折行手术治疗时 ,应考虑到原始骨血肿丢失对骨折愈合的影响 ,术中注意保存部分原始骨血肿可能有利于骨折。

Objective To explore the osteogenetic potential of the primitive bone hematomas by cold storage after fracture.Methods Firstly the bone defect models were built in the femur and tibia of the rabbits of New Zealand.Three days later,take out the primitive bone hematomas and refrigerating them for three days,then bone hematoma was implanted back to the bone defect sections.Finally their osteogenetic potency was observed by regular photograph and histology.Results More osteotylus and periosteal reactions can be observed by regular photograph (after migrating 2,4 and 6 weeks),and osteoblasts can be found by histology in the bone detect sections of the experimental group.Conclusion The primitive bone hematomas have a good osteogenetic potency and can accelerate bone healing better.So when treated bone fracture by operation,especially for closed fracture,loss of the primitive bone hematomas,should be avoided,and reserving the primitive hematomas would help bone fracture heal up.