摘要
补骨脂素在长波紫外光照射下与核酸分子的碱基发生共价结合。本实验对生物素化补骨脂素标记核酸的条件和效果进行了研究,发现该试剂不仅能标记双链DNA、单链DNA,更重要的是可以直接标记RNA及寡核苷酸。探针显色灵敏度可达1pg,杂交灵敏度可达2～5pg。
Covalent addition between psoralens and pyrimidine bases of nucletide is effected by controlledirradiation.We have systematically studied the relations between the probe labelling effects and conditions,and found that it not only can label dsDNA, ssDNA, but also RNA and oligonucleotide. The probehas a colorimetric sensitivity about 1pg, and a hybridition sensitivity to about 2-5pg.
出处
《北京医科大学学报》
CSCD
1993年第2期91-92,共2页
Journal of Peking University(Health Sciences)
关键词
生物素化
补骨脂素
核酸探针
Biotin-psoralen
Nucleic acid probes
Sensitivity
