ITP患者外周血淋巴细胞共刺激分子表达及意义

Expression of costimulatory molecules on peripheral lymphocytes in patients with idiopathic thrombocytopenic purura

目的 研究共刺激分子在特发性血小板减少性紫癜 (ITP)外周血淋巴细胞的表达 ,并探讨其发病机制。方法 应用流式细胞术检测 2 8例 ITP患者及 15例正常对照者的外周血淋巴细胞 CD80 、CD2 8、CD86 表达 ,用酶联免疫吸附法检测血小板相关抗体 (PAIg G)水平 ;并与患者的临床资料进行相关性分析。结果 1ITP组外周血淋巴细胞 CD2 8表达率降低 ,CD80 表达率略增加 ,与对照组均无统计学差异 ;CD86 表达率明显高于对照组 (P<0 .0 1)。 2 ITP组 2 1例 PAIg G水平升高 ,均值为 (197.39± 6 7.81) ng/ 10 7PA。 3ITP组外周血淋巴细胞 CD86 表达率与其巨核细胞数值呈正相关 (P<0 .0 5 )。结论 ITP患者外周血淋巴细胞共刺激分子 CD2 8、CD80 表达无缺陷 ;CD86 表达明显增加。表明 CD86 可能参与 ITP的发病机制 ,应用抗 CD86 单克隆抗体方法可能治疗 ITP。

Objective To investigate the expression of costimulatory molecules on peripheral lymphocytes in patients with idiopathic thrombocytopenic purura(ITP) in order to research the pathogenesis of ITP. Methods The expression of costimulatory molecules on the peripheral lymphocytes was measured by flow cytometry in 28 ITP patients and 15 normal subjects.The expression of PAIgG of ITP patients was analysed by ELISA method.Results The expression of costimulatory molecules of CD 28 and CD 80 on the surface of peripheral lymphocytes was normal in ITP patients.The expression of costimulatory molecule CD 86 was higher in ITP patients than that in normal controls(P<0.01).The PAIgG level was higher in 21 ITP cases with a mean of(197.39± 67.81)ng/10 7 PA.A positive correlation was found between the expression of CD 86 and the number of megakaryocyte(r= 0.20,P<0.05).Conclusion The expression of CD 86 on the surface of peripheral lymphocytes in ITP patients increased and it might be related to the pathogenesis of ITP.Our research may provide a new way in the treatment of ITP with monoclonal antibody.