肿瘤坏死因子-α-863位点基因多态性与心绞痛的相关性研究

The relationship between tumor necrosis factor-α-863 site polymorphism and angina pectoris in Chinese

目的 了解湖北地区汉族人群心绞痛患者肿瘤坏死因子 -α(TNF-α) - 86 3位点基因型的分布特点。方法 应用聚合酶链反应 -限制性片段长度多态性 (PCR- RFL P)技术检测 78例心绞痛患者和 115例正常人的TNF- α基因型。结果 TNF- α- 86 3位点基因型 CC、CA+AA频率在患者组和对照组分别为 80 .8%、19.2 %和6 2 .6 %、37.4 % ;等位基因 C、A频率在患者组和对照组分别为 89.7%、10 .3%和 80 .4 %、19.6 %。两组基因型和等位基因频率比较均有显著性差异 (P<0 .0 5 )。结论 TNF-α- 86 3位点基因多态性与心绞痛有相关性 。

Objective To investigate the relationship between tumor necrosis factor-α(TNF-α) -863 site polymorphism and angina pectoris in Chinese.Mothods The polymorphism of TNF-α gene was screened by PCR-RFLP.Results TNF-α -863 genotype frequencies of CC,CA+AA were 62.6%,80.8% and 37.4%,19.2% in angina pectoris and control group respectively.Allele frequencies of C,A were 89.7%,10.3%和80.4%,19.6%,respectively.There was significant difference in frequencies of genotype and allele in TNF-α -863 polymorphism between two groups (P<0.05).Conclusion TNF-α -863 polymorphism is associated with angina pectoris,C allele may be a risk factor in the processing of angina pectoris in Chinese.