核转录因子-κB及细胞间粘附分子-1在大鼠视网膜缺血再灌注损伤中的表达及吡咯醛二硫氨基甲酸酯对两者表达的影响

Expression of NF-κB and ICAM-1 in rat′s retina injured by ischemia-reperfusion and the effect of pyrrolidine dithiocarbamate on the expression

目的 探讨核转录因子 - κB(NF- κB)及细胞间粘附分子 (ICAM) - 1在大鼠视网膜缺血再灌注损伤中的表达及吡咯醛二硫氨基甲酸酯 (PDTC)对两者表达的影响。 方法 建立大鼠视网膜缺血再灌注模型 ,6 0只 SD大鼠随机分为缺血再灌注组和缺血再灌注 +PDTC治疗组 ,每组 30只大鼠。每只大鼠结扎左侧颈总动脉 ,右侧未作结扎作为对照。每组按再灌注后不同时间段再分为 1、6、12、2 4、4 8、72 h组 ,每组5只大鼠。以原位杂交法检测视网膜中 NF- κB和 ICAM- 1的表达 ,每只大鼠在 1、6、12、2 4、4 8、72 h相应时间段行断颈处死前均行视网膜电图 (ERG)检测 ,并分别计算出左眼或右眼 ERG a、b波波幅的比值 ,得出ERG a、b波的相对恢复率。 结果 缺血再灌注组在再灌注后 6 h开始检测到 NF- κB和 ICAM- 1的表达 ,在 2 4 h表达最强 ,以后逐渐减弱。缺血再灌注 +PDTC组在再灌注后 6 h未检测到 NF-κB和 ICAM- 1的表达 ,在 12 h有 NF-κB和 ICAM- 1的表达 ,2 4 h表达最强 ,但低于缺血再灌注组。对照组未检测到 NF-κB和ICAM- 1的表达。缺血再灌注各组 ERG a、b波波幅相对恢复率低于缺血再灌注 +PDTC组 (P<0 .0 1)。缺血再灌注与缺血再灌注 +PDTC组各时间段 ERG a、b波波幅相对恢复率以再灌注后 2 4 h最差 (P<0 .0 1)?

Objective To investigate the expression of nuclear factor(NF)-κB and intercellular adhesion molecule (ICAM)-1 in rat′s retina injured by ischemia-reperfusion, and the effect of pyrrolidine dithiocarbamate (PDTC) on the expression of NF-κB and ICAM-1. Methods The model of retinal ischemia-reperfusion was set up in 60 SD rats, which were divided into two groups with 30 rats in each: ischemia-reperfusion group and ischemia-reperfussion with injection of PDTC group. The left cephalic artery of each rat was ligated, and the right side was the control. Every group was subdivided into group 1 hour, 6, 12, 24, 48, and 72 hours after ischemia-reperfusion injury, and with 5 rats in each group. mRNA of NF-κB and ICAM-1 mRNA was measured by in situ hybridization (ISH) method in rat′s retina. Every rat underwent electroretinography (ERG) at the corresponding time before executed by neck breaking. Results In ischemia-reperfusion group, expression of NF-κB and ICAM-1 was detected at the 6th hour after ischemia-reperfusion, reached the highest level at the 24th hour, and weakened gradually later. In ischemia-reperfusion with injection of PDTC group, expression of NF-κB and ICAM-1 was detected at the 12th hour after ischemia-reperfusion, and reached the highest level at the 24th hour but lower than that in ischemia-reperfusion group. No expression of NF-κB and ICAM-1 was found in the control group. The relative recovery rate of ERG a and b wave amplitude in ischemia-reperfusion groups was lower than that in ischemia-reperfusion with injection of PDTC group at every stage(P<0.01). The lowest relative recovery rate of ERG a and b wave amplitude in different stages in both of the 2 groups was at the 24th hour(P<0.01). Conclusions NF-κB and ICAM-1 may play an important role in retinal ischemia-reperfusion injury, as the inhibitor of NF-κB, PDTC may relieve the retinal ischemia-reperfusion injury.