清开灵有效组分对MCAO大鼠脑微血管内皮细胞的影响

Effects of Effective Component from“Qing Kai Ling”on Endothelial Cell of Microvessel in MCAO Rats

目的:观察脑缺血后不同时间段MCAO大鼠血浆ET-1、TXB_2、6-keto-PGF_1α、vWF表达水平及清开灵注射液有效组分黄芩苷、栀子苷、胆酸、珍珠母水解液对其表达的影响,探讨不同组分对脑缺血后微血管内皮功能状态干预的作用环节。方法:采用大鼠MCAO模型,用放免法检测脑缺血12h及24h血浆ET-1、TXB_2、6-keto-PGF_1α含量,ELISA法检测不同时段血浆vWF的含量。结果:血浆ET-1含量在不同时段均升高,各有效组分于缺血12h未表现有意义的生物效应,合方、栀子苷阻抑缺血24h血浆ET-1高表达;血浆TXB_2含量在缺血12h显著升高,除合方外有效组分均有阻抑其高表达作用,其中珍珠母作用更显著,至缺血24h,MCAO大鼠TXB_2表达与正常组差异不显著,黄芩苷、合方能较强的抑制其表达;血浆6-keto-PGF_1α含量在缺血后两个时段均显著降低,黄芩苷可升高缺血12h血浆6-keto-PGF_1α水平,珍珠母可升高缺血24h血浆6-keto-PGF_1α水平,胆酸于两个时段均发挥有意义的生物效应;TXB_2/6-keto-PGF_1α比值在不同时段均显著升高,栀子苷、胆酸、黄芩苷、合方能降低缺血12h其比值,黄芩苷、合方能降低而栀子苷可升高缺血后24h其比值;血浆vWF水平于缺血后不同时间段均显著升高,珍珠母在不同时段均有阻抑作用,黄芩苷阻抑缺血12h vWF释放。

Objective:The plasma expression levels of ET-1, TXB_2,6-keto-PGF_1α, vWF at different time after cerebral ischemia were assayed for observing the effects of baicalin, jasminoidin, cholalic acid, hydrolysis fluid of nacre and the combined prescription(CP) on cerebral vasoconstriction and endothelial cells in MCAO rats. Methods: The plasma levels of ET-1, TXB_2,6-keto-PGF_1α in MCAO rats were detected by the method of RIA and the plasma expressions of vWF were observed by ELISA. Results:The levels of ET-1, TXB_2/6-keto-PGF_1α and vWF all increased at different time after cerebral ischemia,so do TXB_2 at 12 hours after ischemia. The expression of 6-keto-PGF_1α significantly reduced at different time point after ischemia in MCAO rat. There were no significant changes after medicine treating 12 hours except baicalin's increasing 6-keto-PGF_1α level. Jasminoidin and CP significantly reduced the expression of ET-1 at 24 hours after ischemia, so do all effective components except CP on expression of TXB_2 at 12 hours after ischemia. The expression of TXB_2, was significantly decreased by baicalin and CP at 24 hours after cerebral ischemia. Both baicalin and cholalic acid significantly increased the expression of 6-keto-PGF_1α at 12 hours after ischemia while cholalic acid and hydrolysis fluid of nacre increased its level after ischemia for 24 hours. TXB_2/6-keto-PGF_1α ratio was reduced distinctively by baicalin, jasminoidin, cholalic acid, CP at the point of 12 hours, while decreased by baicalin and CP, and increased by jasmionoidin at the point of 24 hours. On the other hand,baicalin, hydrolysis fluid of nacre significantly reduced and jasminoidin increased the expression of vWF at the point of 12 hours. At the point of 24 hours, expression of vWF reduced by hydrolysis fluid of nacre and increased by baicalin. Conclusions: The higher plasma expression of ET-1, TXA_2 in plasma aggravated cerebral vasoconstriction and damaged endothelial cells. At the same time, the effective components of 'Qing Kai Ling' inhibit the expression of ET-1, TXA_2 and reduce both TXB_2/6-keto-PGF_1α ratio and level of vWF. As a result,they relax cerebral microvessel and protect endothelial cells by different pathway at different target points.