瑞香狼毒系统溶剂法分离提取部位的体外抗肿瘤活性

The Antitumor Activities of the Extracts from Stellera chamaejasmel L. in Vitro by Means of Systematic Solvent Extraction

目的:分离筛选瑞香狼毒中抗肿瘤活性部位。方法:用系统溶剂法对瑞香狼毒95％乙醇提取物进行分离,MTT比色法对分离得到的部位进行体外抗肿瘤活性的测定。结果:石油醚提取物和氯仿提取物对体外培养肿瘤细胞表现出较强的抑制作用,在测定浓度范围内呈现出良好剂量依赖性抑制作用,而正丁醇和甲醇提取部位在测定浓度范围内则显示出很弱的抑制作用;在各提取部位中石油醚提取物的体外抗肿瘤作用最强。结论:石油醚提取部位是瑞香狼毒中的体外抗肿瘤活性部位。

Objective:To screen the antitumor extract from Stellera chamaejasmel L.. Methods:The means of systematic solvent extraction was used to separate the 95% ethanol extract. The antitumor activities of the different extracts in vitro were analyzed with MTT assay. Results:The petroleum ether extract and the chloroform extract strongly inhibited cell proliferation of human cancer cell lines such as leukemia HL-60, stomach cancer SGC-7901 and hepatocarcinoma BEL-7402,and showed significant dose-dependent response while the n-butanol extract and the methanol extract exhibited low antitumor activities in vitro. The petroleum ether extract was most active. Conclusion:The petroleum ether extract is the principal antitumor extract from Stellera chamaeiasmel L..