银杏外种皮多糖对HL-60细胞的体外实验研究

Experimental Study of Ginkgo biloba Exocarp Polysaccharides on HL-60 Cells in Vitro

目的:研究银杏外种皮多糖对体外HL-60细胞的作用。方法:运用MTT方法和流式细胞技术,检测HL-60细胞体外增殖、凋亡以及相关基因的表达。结果:银杏外种皮多糖(40～160mg/L,48h)可抑制HL-60细胞增殖及增殖促进基因c-myc的表达,可诱导HL-60细胞凋亡并下调凋亡抑制基因bcl-2的表达。结论:银杏外种皮多糖对HL-60细胞增殖及凋亡的影响涉及增殖促进基因c-myc和凋亡抑制基因bcl-2。

Objective:To study the effect of Ginkgo biloba exocarp polysaccharides(GBEP) on HL-60 cells in vitro. Method:Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of HL-60 cells. Result:GBEP(40～160mg/L,48h) could inhibit HL-60 cells proliferation and the expression of proliferation-promotion gene cmyc, induce HL-60 cells apoptosis and down-regulate the expression of apoptosis-inhibitory gene bcl-2. Conclusion: Influence of GBEP on HL-60 cells proliferation and apoptosis may relate to its effects on the expression of proliferation-promotion gene c-myc and apoptosis-inhibitory gene bcl-2.