氧化低密度脂蛋白内皮受体-1在糖尿病大鼠肾皮质的基因表达

Gene expression of lectin-like oxidized low-density lipoprotein receptor in renal cortex of diabetic rat

目的 采用RT PCR法反转录链脲佐菌素 (STZ)诱导的糖尿病 (DM )大鼠肾皮质中氧化低密度脂蛋白内皮受体 (LOX 1)基因 ,并以半定量法测定其mRNA含量 ,探讨其在糖尿病肾病 (DN)的发生和发展中可能参与的机制。方法 选择 18只成年SD大鼠 ,注射STZ制备DM模型 ,14只为DM组 ,4只注射胰岛素作为治疗对照组 (TC组 ) ,另选 4只作为正常对照组 (NC组 )。每 4周测定尿白蛋白定量 ,14周取肾皮质匀浆后采用RT PCR检测其中LOX 1mRNA的表达 ,并采用半定量法测定其含量变化。结果 DM组肾皮质内LOX 1mRNA含量为 5 7.82± 9.37,显著高于NC组 (4 1.0 5± 1.90 ) ,与TC组 (6 0 .2 2± 9.84 )的差异无显著性 ;DM组的LOX 1mRNA的相对含量与尿白蛋白定量成正相关 (r =0 .85 0 6 ,P <0 .0 1)。结论 DM大鼠肾皮质内LOX 1的mRNA表达较正常对照组显著上调。且与尿白蛋白排泄存在相关性。提示LOX 1参与DN的发生及发展。

Objective To estimate the mRNA of LOX 1 in the kidney of streptozotocin induced diabetic rat, and its role in the development and progression of diabetic nephropathy. Methods 18 male Sprague Dawley rats received injections of with streptozotocin to induce diabetes 4 given insulin as the therapeutic control group, and the other 14 rats served as the diabetic group. The urine albumin was guantitatively determined every four weeks, 14 weeks later, and the expression of LOX 1 mRNA in the renal cortex of the rats was detected by RT PCR 14 weeks later. Results The LOX 1 mRNA increased significantly in the kidneys of the diabetic group vs the control group(57.82 ±9.37 vs 41.05±1.90, P <0.05), but it was not decreased significantly in the group receiving insulin treatment (60.22± 9.84, P >0.05). The urine albumin content was related to the increase of LOX 1( r =0.850 6, P< 0.01 ).Conclusions LOX 1 is upregulated in diabetic kidney, its interaction with Ox LDL may play an important role in the development and progression of diabetic nephropathy.