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Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA mircoarray 被引量:9

Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA mircoarray
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摘要 AIM: To characterize the gene expression profiles associated with activation of mouse hepatic stellate cell (HSC) and provide novel insights into the pathogenesis of hepatic fibrosis. METHODS: Mice HSCs were isolated from BALB/c mice by in situ perfusion of collagenase and pronase and singlestep density Nycodenz gradient. Total RNA and mRNA of quiescent HSC and culture-activated HSC were extracted, quantified and reversely transcripted into cDNA. cDNAs from activated HSC were labeled with Cy5 and cDNAs from the quiescent HSC were labeled with Cy3, which were mixed with equal quantity, then hybridized with cDNA chips containing 4 000 genes. Chips were washed, scanned and analyzed. Increased expression of 4 genes and decreased expression of one gene in activated HSC were confirmed by reverse transcription- polymerase chain reaction (RT-PCR). RESULTS: A total of 835 differentially expressed genes were identified by cDNA chip between activated and quiescent HSC, and 465 genes were highly expressed in activated HSC. The differentially expressed genes included those involved in protein synthesis, cell-cycle regulation, apoptosis, and DNA damage response. CONCLUSION: Many genes implicated in intrahepatic inflammation, fibrosis and proliferation were up-regulated in activated HSC. cDNA microarray is an effective technique in screening for differentially expressed genes betweentwo different situations of the HSC. Further analysis of the obtained genes will help understand the molecular mechanism of activation of HSC and hepatic fibrosis. AIM: To characterize the gene expression profiles associated with activation of mouse hepatic stellate cell (HSC) and provide novel insights into the pathogenesis of hepatic fibrosis. METHODS: Mice HSCs were isolated from BALB/c mice by in situ perfusion of collagenase and pronase and single- step density Nycodenz gradient.Total RNA and mRNA of quiescent HSC and culture-activated HSC were extracted, quantified and reversely transcripted into cDNA.cDNAs from activated HSC were labeled with Cy5 and cDNAs from the quiescent HSC were labeled with Cy3,which were mixed with equal quantity,then hybridized with cDNA chips containing 4 000 genes.Chips were washed,scanned and analyzed.Increased expression of 4 genes and decreased expression of one gene in activated HSC were confirmed by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: A total of 835 differentially expressed genes were identified by cDNA chip between activated and quiescent HSC,and 465 genes were highly expressed in activated HSC.The differentially expressed genes included those involved in protein synthesis,cell-cycle regulation, apoptosis,and DNA damage response. CONCLUSION: Many genes implicated in intrahepatic inflammation,fibrosis and proliferation were up-regulated in activated HSC.cDNA microarray is an effective technique in screening for differentially expressed genes between two different situations of the HSC.Further analysis of the obtained genes will help understand the molecular mechanism of activation of HSC and hepatic fibrosis.
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第11期1600-1607,共8页 世界胃肠病学杂志(英文版)
基金 Supported by the National Natural Science Foundation of China,No.39800054 and No.39700068
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