再生室修复神经缺损实验模型的研究

A study of experimental model of nerve regeneration room for repairing the defect of peripheral nerve

目的 建立甲壳胺膜管再生室修复大鼠坐骨神经缺损的实验模型 ,并观察其修复效果。方法 S -D大鼠 90只 ,随机等分为A、B、C 3组 ,按缺损长度与神经干直径之比造模 (分别为 4、6、8倍 )。根据缺损长度及周径裁剪合适长度的甲壳胺膜任其卷曲成管状 ,神经远、近端各套入 2mm ;对照侧作肌表面旷置。术后 4、8、12周作组织学观察和远端轴突再生率的比较。结果 术后切口无炎症反应 ,A、B组实验侧 12周后溃疡愈合 ,有肌肉收缩现象 ,髓鞘成熟良好 ,再生组织均无胶原纤维增生。对照侧溃疡加重 ,实验侧轴突再生率优于对照侧 (P <0 .0 1)。结论 根据缺损长度与神经干直径的倍数比建立的实验模型使实验结果的可比性好、说服力强、重复性好、简便易行 ,获得的数据误差小。再生室内的微环境受到的干扰小 ,可防止瘢痕侵入 ,促进神经缺损的修复。

Objective To establish experimental model for repairing the rat's nerve defect with chitin membrane conduit and investigate it's repairing effect. Methods 90 S-D rats were randomly divided into 3 groups-A,B,C. The model was made according to the proportion of the length of defect to the diameter of nerve trunk (A:4 scale; B 6 scale; C:8 scale). Proper sizes of chitin membrane was made and curled into conduit according to the length of defect and diameter,the both ends of the nerve were covered by the conduit by 2 mml; The control side was “neglect” as reported. 4,8,12 weeks after operation, morphologic investigation and comparisons of ratio of distal regenerated axons were made. Results The incision had no inflammation after operation. the ulcer of experimental side in group A and B healed up respectively and had muscular contraction, the myelin sheath matured well. there was no collagen in regenerated tissue. The ulcer in the control side deteriorated. The ratio of regenerated axons was better than the compared side ( P <0.01). Conclusion The experimental model made according to the multiple of defect to the diameter of nerve trunk could make the result more comparable and convincing, and easy to repeat. The bias was small. The environment in the conduit was barely interfered, the conduit could prevent scar from invading and made the defect of nerve repair easily. of defect in peripheral nerve and prevent scar invading.