葡萄球菌蛋白A胶体金分子三维构象的原子力显微镜分析

Analysis on three-dimensional molecular conformation of Staphylococal protein A-gold by atomic force microscopy

目的 确定葡萄球菌蛋白A胶体金分子特征性构象。 方法 应用原子力显微镜扫描生理状态下分布在云母表面的葡萄球菌蛋白A胶体金分子 ,并进行理论计算。 结果 葡萄球菌蛋白A胶体金分子为 5 7.0 0nm× 33.0 0nm× 2 1.6 1nm椭圆球形“左轮手枪状”复合物。 结论 原子力显微镜可以在生理状态下直观测定生物大分子纳米尺度结构。葡萄球菌蛋白A胶体金分子的特征性结构可以作为神经细胞膜表面N -甲基 -D -天冬氨酸受体 (NMDAR)原子力显微镜观测的原位标记物。

Objective To determine the characteristic conformation of Staphylococal protein A-gold. Methods The atomic force microscope (AFM) was used to detect Staphylococal protein A-gold on mica under physiological environment in order to make a theoretical calculation. Results The Staphylococal protein A-gold was as an ellipse spherical compound of 57.00 nm×33.00 nm×21.61 nm. Conclusions AFM is a new method to directly observe biomacromolecular structures of the Staphylococal protein A-gold,which can be used as an in situ labeling substance for AFM to detect N-methyle-D-asp receptor on the neuronal membrane.