丁酸钠诱导人脑胶质瘤细胞系SHG-44凋亡的初步探讨

Effects of sodium butyrate on the growth and apoptosis of human glioma cell line SHG-44

目的 :研究短链脂肪酸盐丁酸钠对培养人脑胶质瘤细胞系SHG - 4 4的增殖抑制和凋亡诱导。方法 :通过细胞体外培养的方法 ,以 2mmol/L和 4mmol/L丁酸钠处理SHG - 4 4细胞 ,用MTT比色试验和PCNA免疫组化染色观察细胞增殖 ,电子显微镜、线粒体跨膜电位 (△ψm)检测以鉴定细胞凋亡。 结果 :①SHG - 4 4细胞增殖受到明显抑制 ,PCNA表达减弱 ;②4mmol/L丁酸钠明显诱导SHG - 4 4细胞凋亡 ,电镜下可见细胞膜内陷自行分割包裹碎裂的核片段和部分胞浆 ,脱离细胞主体 ,形成凋亡小体 ;罗丹明 12 3染色示凋亡细胞△ψm下降。 结论 :丁酸钠能抑制人脑胶质瘤SHG - 4 4细胞的增殖 ,4mmol/L丁酸钠能诱导SHG - 4 4细胞发生线粒体途径凋亡。

Objective:To investigate the effects of sodium butyrate,the most important short chain fatty acids,on growth suppression and apoptosis of SHG-44 glioma cell line.Methods:Human glioma SHG-44 cells were treated with 2 mmol/L and 4 mmol/L sodium butyrate.The inhibition of cell growth was assessed by MTT spectropotometric analysis and immunohistochemisty methods.The apoptosis was observed by reverted microscope and transmission electron microscope.And mitochondrial transmembrane potential was detected by rhodamine 123 dyeing,which confirmed that SHG-44 cells suffered a mitochondrial path apoptosis.Results:Sodium butyrate treatment resulted in significant inhibition of cell growth,with proliferating cell nuclear antigen expression weakened.Furthermore,4 mmol/L sodium butyrate could induce the SHG-44 cells apoptosis.It was proved that cellular membrane shrunk,vacuoles existed in plasm,and apoptosis bodies occurred by morphologic observation.In addition,the △ψm was decreased in apoptosis cells after 4 mmol/L sodium butyrate treatment.Conclusion:These results indicate,in malignant glioma SHG-44 cells,sodium butyrate could not only suppress cell growth,but also induce apoptosis by a mitochondrial path.