新生大鼠注射尘螨对其发育后期气道炎症作用的研究

Effects of house dust mite injected in neonate rats on airway inflammation and TH subset in later life

目的 :研究早期接触屋尘螨 (HDM)能否加重后期哮喘发作时的气道炎症及其相关免疫学机理。方法 :新生Wistar大鼠生后即注射HDM提取液 ,制作哮喘模型 ,检测血IL - 4及IFN -γ水平、OVA -IgE水平、支气管肺泡灌洗液 (BALF)中嗜酸性粒细胞 (EOS)计数。结果 :HDM组EOS百分比、血清OVA特异性IgE水平、IL - 4水平分别为 ( 37.0 7± 1.10 ) %、( 0 .2 0 4± 0 .0 2 3)和 ( 78.96± 12 .18) pg/ml,为最高 ,而IFN -γ水平最低 ,为 ( 93.78± 2 .10 ) pg/ml。 结论 :生后早期接触HDM能加重以后气道变态反应性炎症 。

Objective:To study whether early exposure to house dust mite will aggravate airway inflammation as well as the onset of asthma and to explore the relevant mechanism.Methods:Neonate Wistar rats were injected with HDM subcutaneously instantly after birth.After the four times management,they were established as models of asthma with OVA,and bronchoalveolus was lavaged to count percentage of eosinophils.And levels of IL-4,IFN-γ and serum OVA-IgE were also detected.Results:In HDM group,the percentage of EOS,level of serum OVA-IgE and level of IL-4 were (37.07±1.10) %,(0.20±0.023) and (78.96±12.18) pg/ml,respectively,the highest among the three groups,otherwise level of IFN-γ,the lowest,(93.78±2.10)pg/ml.Conclusion:Early exposure to house dust mite after birth could aggravate the level of airway inflammation in later life and increase the level of IgE,which is related with T H1/T H2 imbalance.