轮状病毒DNA疫苗经不同途径接种诱导体内免疫应答研究

Induction of immune responses against rotavirus by intramuscular and intranasal immunization with rotavirus DNA vaccine

目的 :通过研究不同途径接种轮状病毒DNA疫苗所诱导的体内免疫应答 ,寻找其适合的免疫途径。方法 :轮状病毒基因疫苗 pcDNA1/VP7经肌肉注射及鼻粘膜两种途径免疫BALB/c小鼠 ,利用ELISA方法对其诱导产生的体液免疫应答进行测定。结果 :经肌肉注射及鼻粘膜两种途径接种轮状病毒DNA疫苗后 ,均在BALB/c小鼠体内诱导产生了病毒特异性IgG增高 (P <0 .0 5 ) ,而病毒特异性IgA与对照组相比无显著性差异。结论

Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine,pcDNA 1/VP7,then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.