儿童淋巴结肿大疾病中c-myc的检测及其意义

Determination of c-myc gene in children's lymph node swollen diseases and its clinical significance

目的 :探讨儿童淋巴结肿大疾病与c -myc基因表达的关系。方法 :应用SP免疫组化法检测 84例淋巴结肿大患儿肿大淋巴结标本c -myc基因的表达。 结果 :①、炎症、反应性增生、恶性肿瘤 3种类型淋巴结肿大组织中c -myc表达阳性率分别为 15 .6 2 % ( 5 /32 ) ,4 0 .4 8% ( 17/42 )和 80 .0 0 % ( 8/10 ) ,且差异有显著性 ( χ2 =14 .5 9,P <0 .0 5 )。②、反应性增生肿大和恶性肿瘤性肿大淋巴结组织的c -mye表达区域不同。 结论 :检测c -mye基因的表达 ,对鉴别恶性淋巴瘤与非肿瘤性淋巴结疾病 。

Objective:To study the expression and significance of c-myc in pediatric lymph node swollen diseases.Methods:Expression of c-myc gene in swollen lymph node specimens of 84 children with lymph node swollen diseases were determined with SP immunohistochemistry technique.Results:(1)The positivity rates of the c-myc gene of lymphadenitis,reactive hyperplasia (RH) and lymphoma were 15.62%,40.48%,and 80.00%,respectively,and the difference was significant( χ 2=14.59, P <0.05 ).(2)The region of the c-myc expression in RH and malignant lymphoma were different.Conclusion:The immunohistochemical detection of c-myc may be used to differentiate lymphoma from other lymph node swollen diseases,especially from the RH.