大鹏湾海域河豚鱼毒性状况研究

The study of tetrodotoxin of puffer fish in dapeng bay

目的 掌握深圳大鹏湾海域河豚鱼毒性状况。方法 采集渔民在大鹏湾海域捕获的河豚鱼鲜活或冰鲜 ,采用小鼠生物试验法测定样本鱼不同组织的河豚鱼毒素 (TTX)含量。结果 共检测了五种河豚鱼样本 ,92份棕腹刺豚和 5份暗鳍腹刺豚样本鱼均未检出毒性 ;1份园斑腹刺豚、9份星点东方豚和 2 6份弓斑东方豚样本鱼各组织均检出毒性 ,肝脏毒力均值范围为 1 83 0 0～ 5 1 1 75MU/g,卵巢毒力均值范围为 2 1 2 84～ 35 4 1 0MU/g ,皮肤毒力均值范围为 9 2 1～ 38 31MU/g ,精巢毒力均值范围为 1 1 4 0～ 2 5 6 4MU/g,肌肉毒力均值范围为 7 2 3～ 9 1 7MU/g。弓斑东方豚卵巢的毒力在 3～ 4月份明显高于 9～ 1 0月份并高于其他组织。结论 本次检测的大鹏湾产的园斑腹刺豚、星点东方豚和弓斑东方豚均为有毒鱼种 。

Objective To understand the tetrodotoxin of puffer fish in Dapeng Bay. Methods The tetrodotoxin (TTX) in different tissues of live or refrigerated puffer fishes in Dapeng Bay was tested by bioassay using mouse. Results Five kinds of puffer fish samples have been tested. TTX have been detected in Pleuranacanthus sceleratus, Fugu niphobles and fugu ocellatus but not in L.wheeleri and L.gloveri. The average TTX content in liver, ovary, skin, spermary and muscle of these three species were 183 00-511 75 MU/g,212 84-354 10 MU/g, 9 21-38 31 MU/g, 11 40-25 64 MU/g and 7 23-9 17 MU/g, respectively. The ovary TTX content of fugu ocellatus in March and April were much higher than that in September and October. Conclusion L.wheeleri can be considered as a TTX free puffer fish.