抑白升血糖浆对化疗小鼠造血功能和免疫功能的影响

Effects of YBSX Syrup on hematopoietic and immunologic functions in mice treated by chemotherapy

目的 观察抑白升血糖浆 (YBSX)对环磷酰胺 (CTX)处理的小鼠造血功能和免疫功能的影响并确定其最佳给药方式。方法 将 YBSX按照单纯预防、单纯治疗和预防 +治疗 3种给药方式 ig给药 ,常规方法计数小鼠白细胞数、血小板数、骨髓有核细胞数 ,测定脾脂数 ;鸡红细胞吞噬实验测定巨噬细胞 (MΦ)吞噬率和吞噬指数 ;EL ISA法测定血清中肿瘤坏死因子 (TNF- α)含量。结果 YBSX对白细胞数、血小板数、骨髓有核细胞数、脾指数、巨噬细胞吞噬指数和 TNF-α含量都有不同程度的改善。结论 YBSX可改善受 CTX抑制小鼠的造血功能和免疫功能 。

Object To explore the effects of Yibaishengxue (YBSX) Syrup on hematopoietic and immunologic functions of mice pretreated by cyclophosphamide (CTX) and the most proper regime of the administration of YBSX Syrup. Methods YBSX Syrup was ig administered to the prevention group, the treatment group, and the group which combined the prevention and treatment together. The number of blood leucocyte, platelet and bone marrow cell were counted by conventional methods; spleen index was measured by weighing method. Phagocytic rate and index were evaluated by phagocytic effect of peritoneal macrophage (MΦ) on chick erythrocyte, and the content of serum tumor necrosis factor alpha (TNF-α) was assayed by ELISA. Results YBSX Syrup enhanced the count of leucocytes, platelets, and bone marrow cells, improved the level of spleen index and macrophage phagocytic index, and increased the content of TNF-α in serum dose-dependent. Conclusion YBSX Syrup is able to ameliorate the disorders of hematopoietic and immunologic functions induced by CTX, and show the combination of prevention and treatment regime is the best one.