5-羟色胺2A受体在自杀死亡者海马中的表达

The expression of 5-HT_(2A) R in hippocampus of suicide victims

目的 探寻自杀行为的生物学标记 ,为早期识别和干预自杀行为提供客观依据。方法 通过观察自杀死者与非自杀对照之间脑海马 5 -羟色胺 2A受体 (5 -HT2AR)的表达情况 ,评价 5 -HT2AR表达变化与自杀行为的关系。选取 10例自杀死亡者及 10例非自杀死亡者脑标本 ,进行抗人 5 -HT2AR免疫组化染色及苏木素 -伊红染色观察 ,并在LeicaQWin图像处理与分析系统中进行电子计算机辅助灰度图像分析 ,得到反映组织 5 -HT2AR表达强度的半定量资料 ,采用SPSS10 .0软件进行统计分析。结果 经海马免疫组织化学染色和苏木素 -伊红染色观察 ,自杀死亡组与非自杀对照组之间海马的一般组织学特征无差异。 5 -HT2AR表达的计算机灰度分析显示 :自杀死亡组海马CA1区、CA2区、CA3区及CA4区的受体表达均明显强于非自杀对照组的相应区域 (P <0 .0 1) ,2组齿状回 (DG) 5 -HT2AR的表达无差异 (P >0 .0 5 )。结论 自杀死亡者海马各区 5 -HT2AR表达明显增高 ,海马 5 -HT2AR表达的增高可能是自杀行为的生物学标记。

Objective To explore the relationship between the expression of 5-HT 2A receptor and suicidal behavior, the expression of 5-HT 2A receptor in different hippocampus areas were observed both in suicide victims and normal controls. Methods The brain specimens of 10 suicide victims and 10 normal subjects were obtained from the Forensic Department of Xuzhou Medical College and the Police Station of Xuzhou City,Jiangsu Province. The tissue blocks of hippocampus were processed routinely to prepare paraffin sections, which were divided into two groups: one for HE staining, the other for immunohistochemistric staining to detect the expression of 5-HT 2A receptors. The immunohistochemistric stained sections were studied by using Leica Q Win image processing and analysis system, with the statistics proceeded in SPSS 10.0. Results No differences in histological characters of the hippocampus were found between suicide victims and control subjects in HE stained sections. The expression of 5-HT 2A receptors in different areas (CA1,CA2,CA3 and CA4) were significantly higher in suicide victims than in controls (P<0.01), though there was no difference in DG. Conclusion The expression of 5-HT 2A R in hippocampus is significantly higher in suicide victims than in controls. The high expression of 5-HT 2A receptors may be a biological marker of complete suicide.