PPP1R3基因Asp905Tyr多态性与安徽省汉族人群2型糖尿病相关性研究

Study on the association of PPP1R3 gene polymorphism with type 2 diabetes in Han population of Anhui province

目的 研究1型蛋白磷酸酶的骨骼肌特异糖原靶向调节亚单位基因(PPP1R3)Asp905Tyr多态性与安徽省汉族人群2型糖尿病相关性。方法 选取安徽省合肥地区汉族2型糖尿病患者262例,健康成人104名,运用聚合酶链反应限制性片段长度多态性技术(PCR-RFLP)进行基因型测定。以体重指数(BMI)25kl/m^2为分割点,将病例组和对照组进行分层分析。结果 PPP1R3基因Asp905Tyr多态性与安徽省汉族人群2型糖尿病没有明显的相关性;以BMI<25kg/m^2基因型Tyr/Tyr组为参照组,BMI≥25kg/m^2携有Asp 905等位基因个体的糖尿病发病风险明显增加(OR=3.69,95％CI:1.38～8.89,P=0.006)。结论 PPP1R3基因Asp 905Tyr多态性可能不是安徽省汉族人群2型糖尿病主要的致病因素。肥胖与Asp905等位基因间的交互作用可增加糖尿病的发病风险。

Objective To study the association of muscle-specific glycogen-targeting regulatory subunit of the glucoge-bound protein phosphatase 1(PPPIR3) gene codon 905 Asp/Tyr polymorphism with type 2 diabetes in Chinese Han population in Hefei region of Anhui province. Methods PPP1R3 gene Asp905Tyr polymorphism was detected by polymerase chain reaction and approoriate restriction enzyme (PCR-RFLP) in 262 type 2 diabetic case and 104 normal controls. Case and control groups were divided into subgroups by body mass index (BMI) 25 kg/m^2 Results When PPP1R3 gene Asp905Tyr polymorphism was not associated with type 2 diabetes mellitus. When subjects with BMI<25 kg/m^2 and Tyr/Tyr genotypes were used as reference. Subjects with Asp905 and BMI≥25 kg/m^2 had a 3. 69-fold incrase of risk suffering from type 2 diabetes (OR=3.69,95% CI: 1.38-8. 89, P=0.006). Conclusions PPP1R3 gene Asp905Tyr polymorphism did not seem to play a critical role in the development of type 2 diabetes mellitus in Han population of Chinese in Anhui province but interaction between the Asp 905 and BMI cause the increase of risk of type 2 diabetes.