瑞香狼毒石油醚提取物硅胶柱层析分离组分的体外抗肿瘤作用

In vitro antitumor activities of the fractions of the petroleum ether extract primarily separated from Stellera Chamaejasmel L. by column chromatography on silica gel

目的 :分离筛选瑞香狼毒石油醚提取物中抗肿瘤活性成分。方法 :用硅胶柱层析的方法对瑞香狼毒的石油醚提取物进行分离 ,MTT比色法对分离得到洗脱成分进行体外抗肿瘤活性的测定。结果 :洗脱组分a1和a2 对体外培养的人肿瘤细胞表现出较强的抑制作用 ,在测定浓度范围内呈现出良好剂量依赖性 ,而a4则未显示出抑制肿瘤细胞生长活性。结论 :抗肿瘤活性较高的成分主要在石油醚 -乙酸乙酯的洗脱组分中。

Objective: To screen the antitumor activity of the petroleum ether extract separated from Stellera Chamaejasmel L.. Methods: The column chromatography on silica gel was used to separate the petroleum ether extract fractions a 1 to a 4. The in vitro antitumor activities of the different fractions were analyzed with MTT assay. Results: The fractions of a 1 and a 2 strongly inhibited cell proliferation of human tumor cell lines such as leukemia HL-60, stomach cancer SGC-7901 and hepatocarcinoma BEL-7402, and showed significant dose-dependent response to inhibit, while the fraction of a 4 exhibited no obvious inhibitory activities. Conclusion: The antitumor components are in the petroleum-ethylacetate fractions.