醒脑开窍针法对脑梗死模型大鼠脑组织c-fos基因表达的影响

Influence of XNKQ acupuncture therapy on c-fos gene expression of encephalon tissue in rats with cerebral infarction

[目的]揭示醒脑开窍针法治疗脑梗死的分子调控机制。[方法]采用Northern杂交技术 ,观察局灶性脑缺血模型大鼠脑组织c-fosmRNA的转录水平及醒脑开窍针法的干预作用。[结果]局灶性脑缺血模型大鼠脑组织c-fos基因的表达因部位和时间的不同而异 ,在皮层不同时程段 ,c-fosmRNA的表达呈上升趋势 ,海马区c-fosmRNA的表达呈下降趋势 ,纹体区在1、3、6h下降 ,24、48h略有上升趋势。醒脑开窍针法能使皮层和纹体c-fosmRNA的表达峰值在各个时段均较模型组增高 ,使海马区1、3hc-fos基因表达峰值降低 ,6、24、48h基因表达上调。[结论]醒脑开窍针法可促使神经细胞对缺血损伤产生不同的适应性变化 ,从而增强脑组织的修复能力 ,加速神经元网络的重建 。

Objective To reveal the molecule-modulating mechanism of XNKQ acupuncture therapy in cerebral infarction treatment.[Methods]Using Northern hybridization technique,c-fos RNA transcription level of encephalon tissue of rats with focal cerebral infarction and intervention function were observed.[Result] The c-fos gene expression of encephalon tissue of rats with focal cerebral infarction was different,depending on different location and time.In different period of time of cortex,the c-fos gene expression assumed a rise tendency,c-fos RNA gene expression of Hippocampi area assumed downtrend.It was tended to decrease at1,3,6h and rise slightly at24,48h in striate body area.XNKQ acupuncture therapy could increase c-fos gene expression peak value of cortex and striate body area in each period of time compared to model group.It could decrease c-fos gene expression peak value of Hippocampi area at1,3h,and increase at6,24,48h.[Conclusion] XNKQ acupuncture therapy can urge nerve cell to produce different suitability change to ischemia damage,and thereby reinforce encephalon tissue rehabilitate capability,and accelerate the reconstruction of neuron network,and recover its common function.