乙肝患者血清标志物HBsAg与HBsAb同时阳性的实验分析

Experimental analyzation of hepatitis B patients' serum markers with both HBsAg and HBsAb positive

目的 探讨乙肝患者血清标志物HBsAg和HBsAb同时阳性原因。方法 采用ELISA方法检测每天受检标本 ,收集HBsAg、HBsAb同时阳性检测结果 ,测定其谷丙转氨酶 (ALT)及总胆红素 (T -Bili)等肝功能项目。结果 HBsAb阳性主要出现在慢性乙肝患者及慢性乙肝携带者 ,“大三阳”与“小三阳”中出现没有明显差异。结论HBsAg、HBsAb同时阳性的模式 ,是由多种原因引起 ,HBsAg亚型双重感染可能是主要原因。

Objective In order to search the reason of Hepatitis B patients' serum markers with both HBsAg and HBsAb positive. Methods Using ELISA to detect the samples with enzyme immunoreactions automatic analyzer,to collect the outcome with both HBsAg and HBsAb positive and detect these samples' liver function index such as alanine aminotransferase(ALT) and total bilirubin(T-bili) by using biochemical automatic analyzer.Results HBsAb(+) emerged mostly in chronic hepatitis B patients and Chronic hepatitis B carriers.No significant difference was noted between the samples with HBsAg(+),HBeAg(+) and anti-HBcAb(+),and those with HBsAg(+),Anti-HBeAb(+) and anti-HBcAb(+).Conclution The pattern with HBsAg(+),HBsAg(+) is caused by several factors.The essential element may be superinfection of subtype virus with HBsAg.