喉鳞状细胞癌组织中Survivin基因与p15和p16基因表达的关系

Expression of Survivin gene and its relationship with expression of p15、p16 proteins in laryngeal squamous cell carcinomas

目的 探讨抑凋亡基因Survivin(SVV)在喉鳞状细胞癌 (laryngealsquamouscellcarcinomas,LSCC)中的表达意义及与抑癌基因p15、p16表达的关系。 方法 采用免疫组化链酶卵白素 生物素复合体 (strepavidin biotincomplex ,SABC)方法检测了 4 8例LSCC组织及 2 4例癌旁组织、2 4例正常喉黏膜组织SVV、p15、p16蛋白的表达并进行统计分析。 结果 SVV蛋白在正常喉黏膜中不表达 ,4 8例LSCC组织中 2 7例SVV蛋白阳性表达 ,占 5 6 3% ,2 4例癌旁组织中 6例SVV蛋白阳性表达 ,占 2 5 0 % SVV蛋白在LSCC组织与癌旁组织中的表达以及SVV在癌旁组织与正常喉黏膜中的表达差异均有显著性 (P <0 0 5 )。SVV蛋白表达与LSCC的发生部位、TNM分期、病理分级、淋巴结转移及预后有关 (P <0 0 5 )。p16蛋白强阳性组、p16蛋白弱阳性组及 p16蛋白阴性组的SVV蛋白阳性率差异有显著性 (P <0 0 0 1) ,且表达呈正相关 (C =0 5 2 )。p15蛋白阳性组与 p15蛋白阴性组的SVV蛋白阳性表达差异无显著性。结论SVV基因在LSCC的发生发展中起重要作用 ,并对LSCC的早期诊断、预后判断有意义。SVV的过度表达与 p16抑癌作用的失去可能在LSCC的发病机制中有协同作用。

Objective To investigate the expression of Survivin(SVV)and its relationship with expression of p15?p16 in laryngeal squamous cell carcinoma(LSCC). Methods Using strep avidin-biotin complex(SABC)method,we examined the expression of SVV,p15 and p16 gene in 48 LSCC tissus samples,24 normal laryngeal mucosa tissus samples and 24 normal laryngeal tissus adjacent to the tumors samples. Results SVV was expression in 27 of 48(56.3%)samples of LSCC and expression in 6 of 24(25.0%)samples of normal laryngeal tissus adjacent to the tumors. Normal laryngeal mocosa tissus samples did not expressed SVV. Overexpression of SVV was related to the tumor site?grade?clinical stage and tumor prognosis(P<0.05).The expression of SVV in LSCC was positive correlated with p16 expression(C=0.52 P<0.001), but not with p15 expression. Conclusion SVV may play a role in the pathway of carcinogenesis and tumor progress. It is feasible for early diagnosis and prognosis estimation. Overexpression of SVV gene and de-activation of antioncogene p16 may be play synergetic roles in the carcinogenesis of LSCC.