直肠癌组织中细胞外信号调节激酶的表达与临床病理特征的相关性

Correlative study between expression of extracellular signal-regulated kinase and clinicopathologic feature in patients with rectal carcinoma

目的 检测细胞外信号调节激酶ERK - 1和ERK - 2在不同临床病理时期直肠癌组织中的蛋白表达水平 ,研究它们在直肠癌发生发展中的意义及其与转移的关系。方法 采用蛋白印迹法检测 86例不同临床病理时期直肠癌组织中ERK - 1和ERK - 2蛋白的表达情况 ,分析ERK - 1和ERK - 2在不同临床分期直肠癌中的表达差异 ,并对有无转移直肠癌的ERK - 1和ERK - 2蛋白表达水平进行对比。结果 直肠癌中ERK - 1和ERK - 2的蛋白表达水平高于正常肠黏膜 ,随着直肠癌病期的发展 ,ERK - 1和ERK - 2的蛋白表达水平逐渐增高 ,即D >C >B >A ;有淋巴结或者肝转移的直肠癌 ,ERK - 1和ERK - 2的蛋白表达水平较高。结论 ERK - 1和ERK - 2在直肠癌中呈现高表达 ,随着直肠癌的发展其表达水平逐渐增高 ,ERK可能成为判断直肠癌临床病期和预测直肠癌预后的良好指标。

Objective To investigate the expression of extracellular signal-regulated kinase(ERK) in human rectal carcinoma and to evaluate the relationship between ERK protein level and clinicopathologic parameters. Methods Western blot was used to measure the expression of ERK-1 and ERK-2 proteins in 86 patients with rectal carcinoma. Results ERK-1 and ERK-2 protein levels increased in rectal carcinoma in comparison to normal tissues. Overexpression of ERK-1 and ERK-2 was correlated to clinicopathologic staging, lymph node involvement and hepatic metastases. Conclusion Overexpression of ERK may play an important role in the development of rectal carcinoma, ERK protein level is an important reference to evaluate the tumor stage and prognosis.