近交系大鼠小肠移植模型排斥反应的动态观察

Continuous observation to rejection following small bowel transplantation in inbred rats

目的 :动态观察近交系大鼠小肠移植排斥反应发生规律 ,探讨该模型对小肠移植排斥反应研究的价值。方法 :选用近交系大鼠F344 (RT11)和BN(RT1n) ,根据改良Monchik法建立下腔静脉回流式异位全小肠移植模型。实验分为同系移植组 (F344→F344 ,ITx 组 )和同种移植组 (BN→F344 ,ATx 组 ) ,每组各制作实验模型 8只。结果 :①ITx 组大鼠平均生存期 30d以上 ,ATx 组大鼠平均存活 12d。②ITx 组大鼠术后各时点的大体观察无明显差异 ,而移植小肠病理组织学POD 3d呈非特异性炎症反应表现 ,POD 0、5、7、9d与正常小肠的组织学特征基本相同。③IL - 2Rα和γ INFmRNA转录水平显著性改变早于排斥反应的病理诊断。④ATx 组在POD 5、7、9d的大体表现与组织病理改变分别符合轻、中、重度排斥反应的诊断标准。结论 :近交系大鼠BN→F344与近交系大鼠F344→Wistar适用于小肠移植排斥反应实验研究。

Objective: To explore the values for rejection research of the models through observing rejection rulers following small bowel transplantation(SBT)in Inbred strain rats.Mothods: Heterotopic SBT models were established with Inbred strain F344/RT11 and BN/RT1n by microsurgical technique according to Monchik method,including Isotransplantation(F344→F344,n=8)and allotransplantation(BN→F344, n=8).Results: 1.The mean living time was over 30 days in IT x group ,which was 12 days in AT x group.2.The general conditions have no significant difference on various time points of postoperative day(POD)in IT x group,while pathological features of grafts were concided with diagnosis of nonspecific slight inflammation on POD3 and were the same as the normal grafts on POD 0?5?7 and 9.3.Significant difference of IL-2Rα chain and γ-INF mRNA transcription was earlier than pathological feature appearance of rejection.4.The general conditions and pathological features of grafts were concided with diagnosis of mild,moderate and severe actute rejection in AT x group on POD 5?7and 9 respectively.Conclusion:Inbred strain BN to F344 combination is fitter for research on rejection mechanism than F344 to Wistar combination and Outbred strain Wistar to SD combination.