核因子-κBp65及bcl-x_L基因在胰腺癌组织中表达及意义

The expression of NF-κBp65 and bcl-x_ L gene in pancreatic carcinoma and their significance

目的 :探讨核因子 κB(NF κBp6 5 )及凋亡相关基因bcl xL 在胰腺癌组织中的表达及其意义。方法 :应用免疫组织化学SABC法 ,检测 5 2例胰腺癌组织中NF κBp6 5及凋亡相关基因bcl XL 的基因蛋白产物 ,并分析其与胰腺癌临床病理的关系。结果 :胰腺癌组织中NF κBp6 5及bcl XL 的表达率为 6 3 5 %和 73 1% ,显著高于胰腺正常组织 (P <0 0 1)。NF κΒp6 5的表达与肿瘤直径、ΤΝΜ分期、淋巴结转移有关 ,而与分化程度、病理类型无关。bcl XL 的表达与TNM分期有关 ,且与NF κBp6 5呈显著相关。 结论 :NF κΒp6 5是一种与胰腺癌有关的癌蛋白 ,可能通过对bcl xL 的上调作用 ,进而影响胰腺癌的发生发展。

Objective:To study the expression of NF-κBp65 and bcl-x L gene in pancreatic carcinoma and their clinical significance.Methods:The expressions of NF-κBp65 and bcl-x L protein in 52 pancreatic carcinoma were studied by immunohistochemistry,and their relationship to clinical pathology were analyzed.Results:The positive rates of NF-κBp65 protein and bcl-x L protein in pancreatic carcinoma were 63.5% and 73.1% respectively,and which were higher than those of normal pancreatic tissues.The expression of NF-κBp65 was related to tumor diameter,TNM stage and lymph node metastasis,and had no relation with the extent of differentiation and type of pathology.There was positive correlation between NF-κ Bp65 and bcl-x L expression.Conclusion:NF-κ Bp65 may be an important oncoprotein,and may have positive regulation on bcl-x L,then promote tumorgenesis.