Survivin的表达与直肠癌细胞凋亡、血管形成及预后的关系

The expression of survivin in rectal carcinoma and its association with tumor cellular apoptosis,angiogenesis and prognosis

目的 :探讨直肠癌凋亡抑制基因Survivin的表达、细胞凋亡及血管形成的关系及临床意义。方法 :用末端脱氧核糖核酸转移酶介导的TUNEL法和免疫组织化学技术检测 5 0例直肠癌细胞凋亡指数(AI)、Survivin的表达及微血管密度 (MVD)。结果 :5 0例直肠癌组织中Survivin阳性表达率 5 6 % (2 8/5 0 ) ,其表达与AI及MVD相关 ,Survivin阴性表达组的 5年生存率 (81 3% )显著优于阳性表达组(5 0 % ;P <0 0 5 ) ;Cox模型多因素分析表明 ,Survivin及AI指数可作为临床预后判断的指标。结论 :凋亡抑制基因Survivin的异常表达与直肠癌细胞凋亡抑制、血管形成及不良预后密切有关。

Objective:To investigate clinical significance of survivin expression,cellular apoptosis and angiogenesis in rectal carcinoma.Methods:The apoptosis in sit rectal carcinoma was identified by the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling(TUNEL)and survivin expression,microvessel density(MVD) was detected by immunohistologic staining in 50 cases of rectal carcinoma.Results:Survivin was expressed in 28 of 50 rectal carcinoma(56%).The correlation was found between survivin expression,cellular apoptosis and angiogenesis.The 5 year survival rate in the group of survivin-negative cases was significant higher than in the group of survivin-positive cases (81.3% vs 50%,P<0.05).In Cox regression analysis,survivin expression and cellular apoptosis index(AI)were indpendent risk factors for prognosis.Conclusion:Survivin,a inhibiting gene of apoptosis,abnormal expression is correlated with apoptosis inhibition,angiogenesis and poor prognosis in rectal carcinoma.