摘要
目的 :研究胃癌组织核基质蛋白与 p16基因上游序列的相关性。 方法 :应用SDS PAGE技术和Southwestern印迹技术 ,对 2 2例胃癌组织、癌旁组织及正常组织的核基质蛋白进行研究。结果 :与正常组织比较 ,胃癌组织 3 0kD、2 8kD的核基质蛋白表达量明显减少 (P <0 .0 5 ) ;不同分化类型及不同临床分期胃癌组织间比较 ,3 0kD、2 8kD核基质蛋白表达量无明显差异 (P >0 .0 5 )。正常组织核基质蛋白与p16基因上游序列的结合主带为 2 8kD、3 0kD、40kD、43kD、5 0kD ,胃癌组织中为 40kD、66kD。胃癌组织与正常组织比较 ,66kD结合阳性信号明显增强 (P <0 .0 5 ) ;而不同分化类型及不同临床分期胃癌组织间 ,66kD结合阳性信号无显著性差异。结论 :胃癌组织中核基质蛋白的改变及 66kD蛋白与p16基因上游区结合量的异常可能是胃癌发生的早期分子事件。
Objective: To study the correlation between the nuclear matrix in gastric cancer and the upstream of p16 gene. Methods: The SDS-PAGE and Southwestern blot techniques were used to study the nuclear matrix proteins of 22 cases of gastric cancer, adjacent cancer tissue and normal gastric tissue. Results: SDS-PAGE showed that the expressional quantity of 30kD, 28kD nuclear matrix proteins in gastric cancer tissues was decreased significantly compared with that in normal gastric tissues,(P<0.05). There was no significant difference in the expressional quantity of 30kD, 28kD nuclear matrix proteins between the various differentiated types and between the various clinical stages of gastric cancer. The bands of Southwestern blot were 28kD, 30kD, 40kD, 43kD, 50kD proteins in normal tissues, and 40kD, 66kD proteins in cancer tissues. The positive signal of 66kD protein binding to the upstream of p16 gene in cancer tissues was significantly higher than that in normal tissues. There was no significant difference between the various differentiated types and between the various clinical stages of gastric cancer tissues. Conclusion: The alteration of nuclear matrix proteins and the binding of 66kD protein to the upstream of p16 gene in gastric carcinoma might be early molecular events in the development of gastric carcinoma.
出处
《河南医学研究》
CAS
2004年第2期106-109,共4页
Henan Medical Research
