牛免疫缺陷病毒(BIV)核心蛋白在大肠杆菌中的表达和鉴定

EXPRESSION AND CHARACTERIZATION OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS ( BIV ) GAG-P26 PROTEIN IN E.COLI

牛免疫缺陷病毒(Bovine immunodeficiency-like virus,BIV)是近年来分离的一株慢病毒,可引起牛的淋巴结病、淋巴细胞增多症、中枢神经系统损伤和进行性消瘦等多种疾病,并可能干扰免疫系统的正常功能。BIV通过接触感染,但不传染人。BIV感染在美国和西欧流行较广,并造成较大的经济损失。

The gene encoding Bovine Immunodeficiency-like virus ( BIV ) gag P26 protein was cloned into E.coli and expressed as a trpE-P26 fusion protein with a molecular weight of 65kD. The yield of the fusion protein was approximately 28% of the total bacterial protein. The fusion protein can be specifically recognized by the antibody present in the serum from cattle infected with BIV. The recombinant gag P26 protein should enable the detection of antibody to BIV.