利用SSR指纹图谱分析大豆花叶病毒(SMV)病抗源的遗传多样性

Genetic diversity analysis of varieties of Glycine max (L.) Merr resistant to Soybean mosaic virus by SSR fingerprints

利用 61个 SSR( simple sequence repeats)引物对筛选出的 1 1 3份高抗大豆花叶病毒 ( SMV)病抗源进行了遗传多样性分析。 1 1 3个抗源产生了 3 87个等位变异 ,平均每个引物可以扩增 6.3 4个等位变异。采用 Nei-Li公式计算相似系数 ,使用 NTSYS-pc2 .1 0 t数据分析软件 ,非加权组平均法进行聚类。分析结果表明抗源间平均相似系数为 0 .2 95 ,说明鉴定的这些抗源遗传差异较大。 1 1 3个抗源被明显地聚为 7类 ,地理来源相同和亲缘关系较近的品种大多聚在一起。相似系数较小、聚在不同类群中的抗源可能携带不同的抗病基因。

The genetic diversity of 113 soybean varieties with high resistance to SMV was evaluated using 61 SSR primers. A total of 387 allelic variants were obtained in the varieties with average 6.34 alleles per SSR locus. Nei-Li genetic similarity coefficients were calculated using the software of NTSYS-pc2.10t and a dendrogram was constructed by the unweighted pair group method of arithmetic average (UPGMA). Mean similarity coefficient among the varieties was up to 0.295, which indicated that genetic difference among them was significant. Based on the genetic similarity coefficient matrix, the varieties were divided into seven groups. The varieties with the same geographical origin and the approximate pedigree were clustered together. The varieties whose genetic similarity coefficients were small and that were clustered in the different group probably carried the different resistant genes.