外周血T细胞亚群及sIL-2R水平与HBV前C区变异相关性研究

Study on the Relationship between the HBV Pre-C Mutant and the Levels of T Subpopulation and sLI-2R

目的 :探讨慢性乙型肝炎患者HBV前C区变异与宿主免疫之间的关系。方法 :采用msPCR法检测 10 2例慢性乙型肝炎患者血清HBVPre C变异 ,并用APAAP法检测T细胞亚群改变 ,用双抗体夹心ELISA法检测血清sIL 2R水平。结果 :在 10 2例HBVDNA阳性CHB患者中HBVPre C 1896变异检出率达 64 % ( 65 /10 2 )。变异株组及野生株组外周血CD4、CD4/CD8比值明显下降 ;CD8明显增高 ,与正常对照组比较差异均具显著性意义 (P <0 0 1)。变异株组CD4、CD4/CD8比值显著低于野生株组 (P <0 0 5 ) ;而CD8显著高于野生株组 (P <0 0 1)。变异株组sIL 2R水平均显著高于野生株组 (P <0 0 1) ,两组水平均显著高于正常对照组 (P <0 0 1)。结论 :变异株组较野生株组存在更为严重的免疫紊乱 ,并通过多种途径激活宿主免疫 ,导致肝损伤。

Objective: To study the relationship between the HBV pre-C mutant and the host immunity.Methods: Mutant specific PCR(msPCR) was employed for detecting pre-C mutant in 102 cases with HBV-results DNA positive. T subpopulations was detemined by APAAP immune-bridge assay; the cytokines (sIL-2R)levels by ELISA.Results: 65 cases had pre-C mutant,the rate was about 64%(65/102). The percentage of CD4 T lymphocyte and the ratio of CD4/CD8 were significantly lower in mutational group than in wild group(P<0.05), but CD8 T lymphocyte was obviously higher(P<0.01). The quantity of sera sIL-2R in mutational group were obviously higher than in wild group. Both groups were obviously higher than contral group.Conclusion: There were more serious distubance in host immunity in mutant group. The mutant type virous could provoke host immunity resulting in severe liver damage.