肝动脉重建对大鼠部分肝移植后移植肝的影响

Significance of arterial reconstruction on liver grafts after partial orthotopic liver transplantation in rats

目的阐明肝动脉重建在大鼠部分肝移植中的意义。方法SD大鼠96只,POLT组行二袖套法切除左外叶和乳头叶的原位部分肝移植n=24,APOLT组行二袖套法切除左外叶和乳头叶的原位部分肝移植,联合肝动脉重建n=24,分别于术后第1,2,4和7天处死后查肝功能、组织学检查及流式细胞仪检测移植肝增殖活性。结果肝功能ALT、TB第一天即开始明显增高,以后逐渐降低,第四天ALT和TB均高于APOLT组,差异有显著性(P<0.05);组织学检查术后可见APOLT组可见更多的二倍体和多倍体肝细胞(P<0.05);第2天和第4天APOLT组增殖指数分别为(33.81±3.45)%和(35.33±2.52)%较POLT组(23.08±4.66)%和(29.79±1.81)%明显增高,差异有显著性(P<0.01)。结论该研究结果初步表明肝动脉吻合可明显改善大鼠部分肝移植移植肝的功能,减轻移植肝的组织学改变,促进部分移植肝的再生。

Objective:To investigate the significance of arterial reconstruction on liver grafts after partial orthotopic liver transplatation in rats.Methods:A total of96rats were randomly divided into2groups:par-tial orthotopic liver transplantation without arterial reconstruction group(POLT)and partial orthotopic liver transplantation with arterial reconstruction group.Liver function,histology and proliferate index(PI)by flow cytometry were detected at1th,2th,4th and7th day after surgery respectively.Results:Alanine aminotrans-ferase(ALT)and total bilirubin(TB)began to elevate from the first day after surgery.ALT and TB in POLT group at fourth day were higher significantly than those in APOLT group(P<0.05).Histological findings indi-cated that more diploid and polyploid hepatocytes could be seen in APOLT group.PI in APOLT group on the second and fourth day were higher than those in POLT group significantly[(33.81±3.45)%,(35.33±2.52)%vs(23.08±4.66)%,(29.79±1.81)%](P<0.01).Conclusions:Arterial reconstruction can improve liver function of liver grafts after POLT,alleviate the histological changes and promote the regeneration of liver grafts.