不同时期的母鼠孕期应激对子代成年后海马的影响

Effects of prenatal stress on different gestational timepoints on mouse hippocampus of adult offspring

目的为了检测不同时期的母鼠孕期应激对子代成年后海马的影响。方法通过被迫游泳试验应激不同孕期的昆明小鼠,利用尼氏染色和Brdu免疫组织化学染色,观察子代成年后海马神经细胞数和神经干细胞的变化。结果孕5天应激的母鼠的子代(P5组)成年后海马CA2和CA3区神经元密度与孕18天应激的母鼠的子代(P18组)相比显著下降(P<0.05);Brdu标记的神经干细胞主要分布于齿状回的颗粒下区,P5组标记的神经干细胞数明显低于正常对照组(P<0.05)。结论孕期应激可影响子代成年后的海马神经干细胞以及相应的海马神经元发生;并且孕期应激对子代的影响与应激时相有关,早期应激比晚期应激的影响可能更大。

Objective:To observe the effects of prenatal stress on the different gestational timepoints on the mouse hippocampus of adult offspring.Methods:Nissl staining and Brdu immunohistochemistry were used in the model of forced swim stressed gestational mice in this study.Results:The neuron density of the P18group is much higher than that of the P5group in the hippocampus CA2and CA3areas(P<0.05);Brdu pos-itive cells were mainly localized in subgranule zone of dentate gyrus,positive cells number in the P5group was lower compared with the normal control(P<0.05).Conclusions:Prenatal stress may indirectly affect the stem cells and relative neurogenesis in the hippocampus of adult offspring;the effects of prenatal stress on the hippocampus of adult offspring is related to the beginning time of stress,the earlier the more serious.