中药“拮新康”对小鼠移植性白血病模型的作用研究

Study on effects of Jie Xin Kang on animal model of murine transplant leukemia

目的探讨拮新康对小鼠移植白血病模型的作用,为临床应用提供实验依据。方法采用急性粒、单核细胞白血病细胞株(WEHI-3B)接种于BALB/C小鼠体内建立移植性白血病模型。然后给小鼠移植性白血病模型采用拮新康不同剂量及长春新碱80进行试验,观察小鼠骨髓未分化细胞、脾脏的重量、脾脏未分化细胞浸润情况。结果拮新康不同剂量实验组小鼠骨髓中未分化细胞较对照组明显减少(P<0.01)。拮新康与长春新碱80联合作用较长春新碱80、拮新康单药组骨髓中未分化细胞明显减少(P<0.01)。拮新康不同剂量实验组小鼠脾重量明显低于模型对照组(P<0.01)脾脏病理所见未分化细胞浸润较对照组减少。结论拮新康对小鼠移植性白血病模型有一定的抑制作用,尤其是与长春新碱80联合抗白血病作用更加增强。

Objective:To explore anti-leukemia efficiency of Jie Xin Kang(JXK)on animal model of murine transplant leukemia and provide evidences for clinical application.Methods:BALB/C mice were inocu-lated of aute myelomonacytic leukemia(WEHI-3B cells).Animal model of transplantation was established.Then,model of murine transplant leukemia was treated with JXK.Undifferentiated cell of bone marrow,splenic infil-tration undifferentiated cell and spleen weight were checked after transplantation.Results:(1)The undifferenti-ated cells numler of bone marrow in variety of doses JXK groups was significantly lower than that in model group of leukemic mice(P<0.01).Undifferentiated cells number of bone marrow in vincristine combined with JXK group was significantly lower than that in JXK,vincristine group,respectively(P<0.01).(2)Spleen weight in experiment group was significantly lower than that in model group of leukemic mice(P<0.01).Infiltration of spleen undifferentiated cell in experiment group was significantly lower than that in control group.Conclusions:Model of leukemic mice can be effectively inhibited by JXK,especially vincristine combined with JXK.