孕期被动吸烟对新生大鼠肺SP-A和SP-B mRNAs表达影响的研究(英文)

Effects of expression of surfactant protein A and B mRNAs in lung of rat pups from maternal passive smoking

目的研究孕期被动吸烟对新生大鼠肺表面活性蛋白A、B(SP-A,SP-B)基因表达的影响,探讨产前被动吸烟对新生大鼠肺损伤的机制。方法分别将SD大鼠于妊娠第1、8和15天置于吸烟箱内每天2h被动吸烟建立孕鼠被动吸烟动物模型,孕鼠每天置于吸烟箱内2h不吸烟者为对照组,于妊娠第20天剖宫取胎鼠,采用反转录聚合酶链反应(RT-PCR)方法检测新生鼠肺组织SP-A、SP-BmRNAs的表达。结果不同孕期被动吸烟SD大鼠所产新生鼠肺组织SP-A、SP-BmRNAs的表达与对照比较和组间无明显差异(P>0.05)。结论孕期被动吸烟对新生大鼠肺组织SP-A和SP-BmRNAs的表达无明显影响。

Objective:To study if prenatal exposure to sidestream cigarette smoke influences the expres-sion of surfactant protein A(SP-A)and surfactant protein B(SP-B)mRNAs in the lung of rat pups.Meth-ods:Sprague-Dawley rats on pregnant day1,8and15were exposed to sidestream cigarette smoke in a whole-body exposure chamber for2h each day.The pups were delivered by cesarean section on the20th day of gestation.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate relative amount of SP-A and SP-B mRNAs expression.Results:The relative amount of SP-A and SP-B mRNAs in the lung of rat pups did not show any significant difference between the control and smoke-exposed groups at any exposure point.Conclusions:The results indicate that prenatal exposure to sidestream smoke does not in-fluence the expression of SP-A and SP-B mRNAs.