硒对大鼠肝细胞凋亡和原癌基因c-myc、c-fos和c-jun表达的影响

Effects of selenium on rat hepatocellular apoptosis,proto-oncogene c-myc, c-fos and c-jun expression

目的 研究亚硒酸钠对大鼠肝细胞凋亡和原癌基因c myc、c fos和c jun表达的影响。方法 选用大鼠 ,每组 5只 ,用 5、10和 2 0 μmol/kg亚硒酸钠腹腔注射染毒。用末端标记法 (TUNEL)、流式细胞术检测细胞凋亡 ,用Northern斑点杂交方法研究原癌基因c myc、c fos和c jun的表达。结果 5、10和 2 0 μmol/kg的亚硒酸钠染毒大鼠 ,不仅能诱导大鼠肝细胞凋亡 ,细胞凋亡率均较对照组 ( 2 2 2± 0 43 ) %显著增高 ,分别为 ( 3 72± 1 76) % (P <0 0 5 )、( 5 82± 1 42 ) % (P <0 0 1)和 ( 11 76± 1 87) % (P <0 0 1) ,而且还存在着明显的剂量 反应关系。 5、10和 2 0 μmol/kg的亚硒酸钠也能引起大鼠肝细胞c myc、c fos和c junmRNA明显增多 ,免疫组化分析可以检测到表达产物c Myc ,c Fos和c Jun蛋白 ,说明这 3种原癌基因表达增强。结论 一定剂量的亚硒酸钠可以诱导大鼠肝细胞凋亡和原癌基因c myc、c fos和c

Objective This study was conducted to explore effects of selenium on rat hepatocellular apoptosis,proto-oncogene c-myc,c-fos and c-jun expression in vivo.Methods Sodium selenite at the doses of 5,10 and 20 μmol/kg were given to rats by i.p.and there were 5 male SD rats in each group. Hepatocellular apoptosis was measured with TdT-mediated dUTP nick end labelling(TUNEL) and flow cytometry. Proto-oncogene c-myc, c-fos and c-jun expression in rat hepatocytes were measured with northern dot hybridization.Results The results showed that sodium selenite at the doses of 5, 10 and 20 μmol/kg, not only induced hepatocellular apoptosis, apoptosis rates were(3.72±1.76)%(P<0.05), (5.82±1.42)%(P<0.01)and(11.76±1.87)%(P<0.01) respectively, but also displayed obvious dose-response relationship. In addition, sodium selenite at these doses resulted in proto-oncogene c-myc, c-fos and c-jun expression significantly, c-Myc,c-Fos and c-Jun protein can be detected in rat hepatocytes with immunohistochemistry.Conclusion selenium at certain doses can induce rat hepatocellular apoptosis,proto-oncogene c-myc, c-fos and c-jun expression significantly in vivo.