单宁急性毒性及其对小鼠丙二醛和抗氧化酶的影响

The acute toxicity of tannic acid and its effect on malondialdehyde and antioxidases in mice

目的 探讨单宁的急性毒性以及其体内抗氧化作用。方法 12 0只二级昆明鼠随机分成 6组 ,设溶剂对照组和 5个单宁处理组 ,分别经一次性灌胃给予剂量为 0 0 0、1 2 5、2 5 0、5 0 0、10 0 0和 2 0 0 0g/kg的单宁水溶液。观察 10d动物的存活情况 ,计算昆明小鼠单宁经口半数致死量 (LD50 )。给药后第 11天 ,处死存活小鼠 ,检测全血过氧化氢酶(CAT)活力、血清和肝脏丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活力。结果 各组动物的死亡率依次分别为 0、0、5 %、5 %、10 %和 85 % ,用改良的寇氏法计算 ,昆明种小鼠单宁经口LD50 为 13 2g/kg ,其 95 %可信限范围为 10 6～ 16 4g/kg。各给药组血清MDA含量较对照组均有不同程度的升高 ,且存在着一定的剂量 效应关系。肝脏MDA变化趋势与血清MDA的变化基本一致。低剂量给药组血清SOD活力较阴性对照组升高 ,而高剂量组血清SOD活力反而降低。单宁给药各组全血CAT活力较对照组降低 ,并且与剂量呈正相关。结论 单宁为实际无毒级 。

Objective To investigate the acute toxicity of tannic acid and its antioxidative effect in vivo.Methods The mice were randomly divided into six groups.Except for the control group,the other five groups were given tannic acid solutions by gavage separately with the dosage of 1.25,2.50, 5.00, 10.00 and 20.00 g/kg body weight. The death ratios were observed and the median lethal dose (LD 50) was calculated.Ten days later, all of the mice were killed to examine the contents of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) in liver and serum.The catalase (CAT) in peripheral blood were also determinated.Results The LD 50 of tannic acid in mice was 13.2 g/kg.The contents MDA increased greatly compared with the control group and had dose-effect relationship with tannic acid.The activities of SOD in serum increased at the dosage of 1.25 g/kg and decreased at other groups.The activities of CAT increased with the dosage of tannic acid.Conclusion The LD 50 of tannic acid in mice is 13.2 g/kg.But the high dosage tannic acid can cause oxidative injury in mice.