珍珠母、胆酸、栀子苷、黄芩苷对局灶性脑缺血大鼠缺血脑组织单核细胞趋化蛋白的影响

Effect of Concha Margatitifera Usta, Cholic Acid, Gardenoside, Baicalin on MCP-1 in the Ischemic Cerebral Tissue of Rats after Middle Cerebral Artery Occlusion

目的 观察缺血过程中珍珠母、胆酸、栀子苷、黄芩苷对大鼠脑缺血后缺血脑组织内单核细胞趋化蛋白(MCP-1)含量的影响。方法 110只雄性SD大鼠随机分为正常组,脑缺血12 h模型组,珍珠母、胆酸、栀子苷、黄芩苷治疗脑缺血12 h组,脑缺血24 h模型组,珍珠母、胆酸、栀子苷、黄芩苷治疗脑缺血24 h组。用ELISA法测定脑缺血12 h后和脑缺血24 h后缺血脑组织MCP-1含量的变化。结果 大鼠局灶性脑缺血12 h后,缺血脑组织MCP-1含量较正常组明显增高(P<0.05)。珍珠母、栀子苷治疗脑缺血12 h组MCP-1含量较缺血12 h模型组明显降低(P<0.01)。胆酸、黄芩苷治疗脑缺血12 h组MCP-1含量较缺血12 h模型组无显著性差异。大鼠局灶性脑缺血24 h后,缺血脑组织MCP-1含量较正常组明显增高(P<0.05)。珍珠母、胆酸、栀子苷、黄芩苷组治疗脑缺血24 h组MCP-1含量较缺血24 h模型组明显降低(P<0.05或P<0.01)。结论 降低缺血后脑组织MCP-1的含量,可能是珍珠母、胆酸、栀子苷、黄芩苷治疗脑缺血的重要机制之一。

Objective To study the effect of Concha Margatitifera Usta, Cholic Acid, Gardenoside, Baicalin on the protein levels of monocyte chemoattractant protein (MCP-1) in the ischemic cerebral tissue of rats after middle cerebral artery occlusion in rats. Methods The 110 male SD rats were randomly assigned to the normal group, middle cerebral artery occlusion for 12 hours group and Concha Margatitifera Usta, Cholic Acid, Gardenoside, Baicalin cure middle cerebral artery occlusion for 12 hours group, middle cerebral artery occlusion for 24 hours group and Concha Margatitifera Usta, Cholic Acid, Gardenoside, Baicalin cure middle cerebral artery occlusion for 24 hours group. The protein levels of MCP-1 were measured by enzyme linked immunosorbent assay (ELISA). Results The protein level of MCP-1 of the ischemic cerebral tissue of rats in the middle cerebral artery occlusion for 12 hours group was markedly increased than the normal group (P<0.05). The protein level of MCP-1 of the ischemic cerebral tissue of rats in Concha Margatitifera Usta and Gardenoside cure middle cerebral artery occlusion for 12 hours group were significantly lower than it in the middle cerebral artery occlusion for 12 hours group (P<0.01). There was no significant change on the protein level of MCP-1 in the ischemic cerebral tissue of rats between Cholic Acid, Baicalin cure middle cerebral artery occlusion for 12 hours group and the middle cerebral artery occlusion for 12 hours group. The protein level of MCP-1 of the ischemic cerebral tissue of rats in the middle cerebral artery occlusion for 24 hours group was markedly increased than the normal group (P<0.05). The protein level of MCP-1 of the ischemic cerebral tissue of rats in the Concha Margatitifera Usta, Cholic Acid, Gardenoside ,Baicalin cure middle cerebral artery occlusion for 24 hours group were significantly lower than it in the middle cerebral artery occlusion for 24 hours group (P<0.05 or P<0.01). Conclusion Reducing the level of MCP-1 of the ischemic cerebral tissue of rats after ischemia may be one of the most important ways for the neuroprotective effects the middle cerebral artery occlusion group.