松材线虫rNDA-ITS1区分子检测与鉴定

Molecular identification of Bursaphelenchus xylophilus by PCR amplification of first internal transcribed spacer of ribosomal DNA

利用两对松材线虫特异性引物,分别对来自日本、美国和葡萄牙的松材线虫虫样进行PCR检测,成功扩增出330 bp和220 bp rDNA-ITS1区基因片段,该方法对松材线虫成虫或幼虫均能作出准确鉴定。

DNA fragments of about 220bp and 330bp were amplified by PCR using two pairs of specific primers that were designed from the ribosomal DNA (rNDA). The genomic DNA of Bursaphelenchus xylophilus and Bursaphelenchus mucronatus originated from Japan, United States and Portugal was used as PCR template. This technique can be used to identify the adults or larval of Bursaphelenchus xylophilus exactly.