太湖底泥表层越冬藻类群落动态的荧光分析法初步研究

Analysis of the Recruitment of the Winter Survival Algae on the Sediments of Lake Taihu by Fluorometry

为探索春季湖泊底泥表层不同藻类群落的复苏规律,本实验采集了太湖梅梁湾地区的底泥,应用荧光分析法测定藻蓝素,同时测定湖泊底泥中的叶绿素a和叶绿素b,确定春季梅梁湾底泥中不同藻类类群的色素含量变化,说明荧光分析法可以应用于底泥色素分析中,初步探讨了蓝藻群落和非蓝藻群落在春季温度上升期间恢复生长过程的差异。

Chlorophyll and its degraded products are often regarded as the indicative marker of the lake productivity. Since the prodominant algae species of the bloom in Lake Taihu is blue algae, the content of phycocyanobilin in the water can indicate the growing level of blue algae in this lake. In order to investigate the roles of the spring recruitment of the algae on the sediment, the surface mud of the sediment samples were collected from Meiliang Bay, Lake Taihu in April and June, 2003. The content of phycocyanobilin was determined by fluorometry, and the contents of chlorophyll a and chlorophyll b were simultaneously determined by synchronous spetrofluorometry. The changes of the pigment contents on the surface of the sediments were confirmed. It is proved that fluorometry can be applied to the analysis of pigments in the sediments because the analytical method is highly sensitive and the results of this experiment are accurate and credible. The dif- ferent characters of the recruitment between blue algae and non--blue--algae were discussed when the temperature increased in spring, which is significant and helpful to the further research on the succession and recruitment of algae.