几种脲酶抑制剂对大豆脲酶和绵羊瘤胃微生物脲酶的抑制作用

Effect of Some Urease Inhibitors on Soybean Urease and Rumen Microbial Urease of Sheep

本文研究了脲酶抑制剂乙酰氧肟酸 (AHA)、邻苯二酚、氢醌 (HQ)和硼砂对大豆脲酶和绵羊瘤胃微生物脲酶的抑制作用。结果表明 ,在浓度为 0 .0 0 0 1,0 .0 0 1,0 .0 1和 0 .1mmol/L时 ,4种脲酶抑制剂对大豆脲酶的抑制率分别为 :AHA为 6 % ,6 .2 % ,9.6 6 %和 2 9.79% ;HQ为 8.4 % ,13.0 3% ,19.79%和 4 4 .75 % ;邻苯二酚为 2 0 .34% ,19.12 % ,83.16 %和 93.78% ;而硼砂为 16 .5 5 % ,17.18% ,18.95 %和 35 .5 0 %。在相同浓度下 ,4种脲酶抑制剂对绵羊瘤胃微生物脲酶的抑制率分别为 :AHA为 9.5 8% ,14 .0 4 % ,4 1.30 %和 72 .73% ;HQ为 12 .2 1% ,39.99% ,6 4 .6 2 %和 78.87% ;邻苯二酚为 6 .0 7% ,9.36 % ,31.2 9%和 5 0 .4 4 % ;而硼砂分别为 4 .97% ,8.6 3% ,2 1.78%和 32 .0 2 %。

Effect of urease inhibitors acetohydroxamic acid (AHA), catechol, hydroquinone (HQ) and borax on rumen microbial urease of sheep and soybean urease were studied in this trial. At concentration of 0.0001, 0.001, 0.01and (0.1) mmol/L of the urease inhibitors, soybean urease activity was inhibited by AHA by 6%, 6.2%, 9.66% and (29.79)%, HQ by 8.4%, 13.03%, 19.79% and 44.75%, catechol by 20.34%, 19.12%, 83.16% and 93.78%, and borax by 16.55%, 17.18%, 18.95% and 35.5%. At the same concentration as above, rumen microbial urease was inhibited by AHA by 9.58%, 14.04%, 41.3% and 72.73%, HQ by 12.21%, 39.99%, 64.62% and (78.87)%, catechol by 6.07%, 9.36%, 31.29% and 50.44%, and borax by 4.97%, 8.63%, 21.78% and 32.02%.