Fe^II(EDTA)络合吸收NO体系中吸收液的生物再生

Microbial Regeneration of Fe^II (EDTA) in Nitric Oxide Removal by Ferrous Complex Absorption

利用驯化的微生物对FeII(EDTA)NO的再生进行了研究。结果表明,驯化过程中,分子量相对较低的乙醇比葡萄糖更适合于作为该体系的碳源;200mgL-1的乙醇添加量可以使9.92mmolL-1FeII(EDTA)NO完全再生,添加过量碳源对反应速率没有促进作用。当FeII(EDTA)NO浓度为7.3mmolL-1,菌体接种量大于80mg(菌体干重)L-1时就能满足再生需要。生物再生的适宜pH值和温度范围分别为6.5~7.5和30~42.5℃;实验考察条件下,该反应符合一级降解动力学,最大反应速率gmax为5.4mmolL-1h-1,半饱和速率常数km为63.8mmolL-1。

A new promising method of NO removal by combined use of microbial regeneration and metal complex absorption was presented. FeII(EDTA)NO will be reduced by microorganisms to the environmentally benign gaseous product of N2, and FeII(EDTA) is regenerated simultaneously. The effects of the type and amount of carbon source, pH value, temperature and the amount of biomass inoculation on bio-reduction efficiency were investigated in the flask culture experiments. The results indicate that the carbon source of ethanol is better than glucose and 200mgL-1 of ethanol is enough for microorganism to reduce 9.92 mmolL-1 FeII(EDTA)NO completely. Reduction rate does not increase with adding more than that amount of carbon source. When the biomass inoculation is more than 80 mgL-1, there will be no 搇ag time?of bio-reduction with an initial FeII(EDTA)NO concentration of 7.3 mmolL-1. The bio-reduction can be achieved efficiently in the temperature range of 30~42.5℃. A pH range of 6.5~7.5 is optimal for achieving effective bio-reduction. The kinetics of the bio-reaction can be described by Michaelis-Menten equation and the maximum reaction rate ggmmaaxx is 5.4mmolL-1hr-1 and the Michaelis constant kkmm is 63.8 mmolL-1 under the certain conditions.