丛生福禄考的离体繁殖

In Vitro Propagation of Phlox subulata L.

建立了丛生福禄考离体繁殖体系。用 0 1%HgCl2 溶液对丛生福禄考的新生嫩茎进行消毒 ,适宜的消毒时间为 10min。 1mm茎尖的成苗率达到 83 3%。去顶芽的试管苗新梢比具顶芽的试管苗新梢的增殖能力高约 5 0 %。采用腋芽萌发途径 ,品种丹尼尔垫和多摩流的增殖系数分别达 18和 8。在附加IBA0 2mg/L的 1/ 2MS培养基上新梢的生根率达到 80 %。试管苗大规模移栽的成活率达 90 %以上。

An efficiency system of propagation in vitro of Phlox subulata L.was developed.The optimum sterilization of young shoots was 10 minutes with 0 1% HgCl 2.The rate of shoots forming from 1 mm shoot tips reached 83 3%.The multiplication coefficient of in vitro decapitated shoot was 50% higher than the multiplication coefficient of whole shoot.For cultivars'Daniel cushion'and'Tamaryuu',the multiplication coefficients reached 18 and 8 respectively.On the half strength MS medium supplemented with IBA 0 2 mg/L,the rate of rooting of in vitro shoots of Phlox subulata L.reached 80%.The survival rate of plants transplanted on a large scale was more than 90%.The efficiency of multiplication of Phlox subulata L.was affected significantly by genotypes.