不同贮藏温度下兰州百合种球淀粉代谢与萌发关系初探

Starch Metabolism and Sprouting of Bulb in Lilium davidii var. unicolor Stored at Different Cold Temperatures

不同的贮藏温度和贮藏时间 ,兰州百合鳞茎碳水化合物含量均有显著差异。顶芽 6℃处理的淀粉含量最高 ,10℃处理最低 ;鳞茎盘的淀粉含量与之相反 ;鳞片的淀粉含量随着贮藏温度的降低而下降。鳞茎各部位可溶性糖的含量以及顶芽和鳞片的淀粉酶活性随着贮藏温度的升高而降低 ,鳞茎盘的淀粉酶活性为 6℃处理最高 ,2℃处理最低。淀粉含量和淀粉酶活性呈极显著负相关。贮藏初期的 34d ,顶芽在鳞茎内迅速发育 ,鳞茎内物质变化最活跃 :淀粉含量明显下降 ,淀粉酶活性迅速增大 ,可溶性糖含量显著升高。顶芽和鳞茎盘的变化幅度大于鳞片 ,外部鳞片的变化幅度大于中部和内部鳞片。贮藏温度与贮藏时间之间的显著互作影响碳水化合物的代谢及鳞茎的萌发。在本试验中 ,2℃贮藏 10

Carbohydrate contents were markedly different according to various storage temperatures, storage durations and parts of bulb. Bulbs stored at 6℃ had the highest starch level in bud and the lowest starch level in basal plate. However, bulbs stored at 10℃ had the opposite trend. Starch content in scales declined along with the decrease of storage temperature. Soluble sugar content in all parts of bulb and amylase activity of bud or scales decreased along with increase of temperature, but showed an order of 6℃>10℃>2℃ in basal plate. An extremely negative correlation was observed between starch content and amylase activity. The first 34 days of cold storage was the most active period of carbohydrate metabolism, which the bud developed rapidly. Total soluble sugar and amylase activity increased in relation with the decrease of starch. The extent of change in bud and basal plate was greater than that in scales,and in exterior scales was greater than in other scales. The significant interaction between temperature and duration affected carbohydrate metabolism and sprouting of bulb. The results indicated that storage at 2℃ for 101 d was the best treatment for the dormancy release.