草莓愈伤组织再生能力与活性氧代谢水平相关性研究

The Possible Relationship between the Regeneration Capacity and Reactive Oxygen Species in the Strawberry Calli

草莓外植体在不同组合培养基上诱导产生 3类愈伤组织 ,Ⅰ型愈伤组织的再生能力较低 ,Ⅱ和Ⅲ型愈伤组织具有较高的再生能力。Ⅱ和Ⅲ型初生愈伤组织H2 O2 的含量分别是Ⅰ型的 5倍和 9倍 ,O·2的释放速率是其 3倍和 4倍 ,并且具有较高的抗氧化酶活性。在继代培养过程中 ,愈伤组织的形态发生能力下降 ,Ⅰ型愈伤组织逐渐失去再生能力 ,同时所有类型愈伤组织中H2 O2 含量缓慢上升 ,O·2 释放速率较快增长 。

Three types of callus,which present different regeneration capacity,were established in the tissue culture of strawberry.The Ⅱ and Ⅲ types of callus possessed higher regeneration capacity while Ⅰ type of callus had much lower.In the freshly induced calli,the Ⅱ and Ⅲ types of callus had 5 fold and 9 fold higher contents of intracellular H 2O 2,and 3 fold and 4 fold higher level of O [BHDFG1.5mm,WK1.5mmW]· 2,compared with the Ⅰ type of callus, respectively .Also,the calli Ⅱ and Ⅲ had much higher activities of antioxidant enzymes than the callus Ⅰ.During the subculture,H 2O 2 level increased gradually and O [BHDFG1.5mm,WK1.5mmW]· 2 rapidly,while the activities of antioxidant enzymes gradually declined.The ability of expression of totipotency in calli decreased,especially the callus Ⅰ lost the ability after 12 week subculture. The results indicated that the reactive oxygen species might play a dual role in the regeneration of strawberry calli.On the one hand,a certain level of ROS may have a positive effect on the regeneration,and on the other hand, high level of ROS is inhibitory for the expression of totipotency in calli.