玉米大斑病的分离、培养及产孢方法的研究

Study on the Methods for the Isolation, Culture and Spore Production of Northern Corn Leaf Blight

对采自云南省宣威市玉米大斑病标样进行分离、培养及产孢,提出了一种简便易行、污染少的单孢分离法。结果表明,该病菌对蔗糖和玉米汁的利用效果较好;燕麦和燕麦桔子汁培养基更有利于菌丝的生长;光暗交替有利于产孢。为该病原菌的深入研究提供了一种简便易行的方法。

Methods of isolation, culture and sporulation for northern corn leaf blight were assessed. The results showed that the method of single spore isolation was simple, practical, less contaminative and highly effective; the fungi grew better on the medium with sucrose and the juice of maize as the carbon source; the medium of oathmeal and oathmeal—organe juice were better for mycelium growth; the alternation of darkness and light favored the sporulation.