Clostridium paraputrificum M-21发酵制氢培养条件研究

Effect of cultivation conditions on hydrogen production by Clostridium paraputrificum M-21

利用类腐败梭状芽孢杆菌M 2 1(ClostridiumparaputrificumM 2 1) ,在 37℃、15 0r min条件下发酵制氢 ,以葡萄糖为碳源 ,蛋白胨为氮源 ,1mol的葡萄糖可以产生 1 0 5mol的氢气 ,最终所产气体中有 70 %H2 和 30 %CO2 (体积百分数 )。最优初始pH范围为 7 0～ 7 5 ,少量乙酸的存在对氢气的生成有促进作用 ,若大量存在 ,会严重抑制菌的生长。在C N质量比为 1 0时 ,所产氢气体积最多 ,1g葡萄糖可产生 75mLH2 (常温常压 )。在高温 83℃下对种菌预处理 30s促进孢子的萌发 ,会缩短发酵产氢的时间。以淀粉为碳源 ,所产氢气体积略微高于葡萄糖 ,1g淀粉可产生 78mLH2 (常温常压 ) ;以蔗糖为碳源 ,所产氢气体积略微低于葡萄糖 ,1g蔗糖可产生 72mLH2 (常温常压 )。该菌不能降解利用羧甲基纤维素 ,木质素磺酸钠 ,及纸浆等。

An obligate anaerobic Clostridium paraputrificum M 21 is capable of utilizing various sugars to produce hydrogen Under the cultivation conditions of 37℃,150 r/min shaking and initial pH of 8 5 in glucose containing medium, the cells yielded 1 05 mol H 2/mol glucose, and the final composition of gas yielded containing 70% hydrogen and 30 % carbon dioxide, and the final pH lowing down to 5 25 The optimum initial pH of the medium was between 7 0 and 7 5, a little acetate in the medium may promote hydrogen releasing, but a large amount of acetate in the medium may inhibit the cells growing; and the opimum ratio of carbon source to nitrogen source( w/w ) was about 1 0 Heat shock treatment to the inoculum prior to the cultivation for 30 seconds at 83℃, enhanced the hydrogen production The bacterium could also utilize starch and sucrose as carbon sources, but had no capability of utilizing carbxymethylcellulose, sodium lignosulphonate and paper pulp for hudrogen production