葡枝根霉NG0305酶催化甾体C_(11)α羟基化的研究

Study on enzymatic C_(11) α-hydroxylation of steroid by Rhizopus stolonifer NG0305

应用本实验室保藏的葡枝根霉RhizopusstoloniferNG0 30 5对甾体化合物烯睾丙内酯 (3 oxo 4 ,6 diene Pregna 17 alpha hydroxy 2 1 carboxylicacidgama lactone)进行酶催化C1 1 α 羟基化反应的研究。研究结果表明 ,菌体培养的碳源供应对菌体所产羟化酶的活力有重要影响。采用葡萄糖和淀粉组合碳源 ,并加入适量的黑曲霉糖化酶的方式 ,解决了葡萄糖抑制的问题 ,并缩短了菌体培养反应时间 ,得到高羟化转化率。酶转化反应 88h后 ,提取吸附在菌丝球内的产物 ,应用液相色谱测定 ,结果表明C1 1 α 羟基化转化率达到了 5 3 0 %。

Study on enzymatic C 11 α hydroxylation of Steroid(3 oxo 4,6 diene Pregna 17 alpha hydroxy 21 carboxylic acid gama lactone) by Rhizopus stolonifer NG0305 which was stored in the central lab of the college has been conducted in the present paper. The results indicated the supplying method of carbon source seriously effecting on the hydroxylase activity. The complex carbon source composed of glucose and starch with amylase of Aspergillus niger has been applied, with which the inhibition on glucose has been relieved and the culture period has been obviously shorten with higher conversion rate of C 11 α hydroxylation. The product extracted from the mycelial pellets had been determinated by HPLC after enzymatic conversion for 88 hours, the determination showed that the conversion rate of C 11 α hydroxylation reaching 53.0%.