几丁质酶基因和β-1,3-葡聚糖酶基因克隆微生物表达和抑菌效果研究

Studies on cloning and expression of the -1, 3-Glucanase and chitinase genes and their potential for fungal resistance

采用PCR扩增的方法,扩增获得几丁质酶和β-1,3-葡聚糖酶的基因,分别将其基因克隆到原核表达载体pET28a(+)上,构建了原核表达载体pETChi和pETGlu。经IPTG诱导后几丁质酶基因和β-1,3-葡聚糖酶基因在大肠杆菌BL21中得到高效表达。通过对连孢(Alternaria alternate)的抑菌实验表明,这两基因的表达产物对此真菌有较强的抵抗能力。

Chitinase and -1, 3-glucanase genes were amplified by PCR procedures and subcloned into an expression vector Pet-28a(+), the prokanyo expression vector of the both genes were constructed and named pETChi and pETGlu, respectively. A SDS-PAGE analysis indicated that chitinase and -1, 3-glucanase genes were expressed highly in E. coli BL21 after induced by IPTG. Challenged with Alternaria alternate showed that chitinase and -1, 3-glucanase genes had good resistance to the pathogenic fungus.